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α-tocopherol solution  (Millipore)


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    Millipore α-tocopherol solution
    THP-1 cells were incubated for 24 h with or without 7KC et 7β-OH (62.5μM) in the presence or absence of Silybum seed oils (SMSO, SESO, and SMCSO) (100 μg/mL) or <t>α-tocopherol</t> (400 μM). The histogram represented the percentage of DHE-positive cells. The results are expressed as mean ±SD of three independent experiments. Different letters indicate significate difference between the groups ( p <0.05, Tukey test, multiple comparative analysis). a: comparison to control; b: comparison to DMSO (0.166%); c: comparison to Eth (0.104%); d: comparison to “DMSO (0.166%) +Eth (0.104%)”; e: comparison to α-tocopherol (400 μM); f: comparison to SMSO (100 μg/mL); g: comparison to SESO (100μg/mL); h: comparison to SMSO (100 μg/mL); i: comparison to 7KC (62.5 μM); j: comparison to “7KC + α-tocopherol (400 μM)”; k: comparison to “7KC + SMSO (100 μg/mL)”; l: comparison to “7KC + SESO (100 μg/mL)”; m: comparison to “7KC + SMCSO (100 μg/mL)”; n: comparison to 7β-OH (62.5 μM); o: comparison to “7β-OH + α-tocopherol (400 μM)”; p: comparison to “7β-OH + SMSO (100 μg/mL)”; q: comparison to “7β-OH + SESO (100 μg/mL)”; r: comparison to “7β-OH + SMCSO (100 μg/mL)”.
    α Tocopherol Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1-tocopherol+solution/%CE%B1+tocopherol+solution/pmc11178192-212-2-4
    Average 90 stars, based on 1 article reviews
    α-tocopherol solution - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests"

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests

    Journal: PLOS ONE

    doi: 10.1371/journal.pone.0304021

    THP-1 cells were incubated for 24 h with or without 7KC et 7β-OH (62.5μM) in the presence or absence of Silybum seed oils (SMSO, SESO, and SMCSO) (100 μg/mL) or α-tocopherol (400 μM). The histogram represented the percentage of DHE-positive cells. The results are expressed as mean ±SD of three independent experiments. Different letters indicate significate difference between the groups ( p <0.05, Tukey test, multiple comparative analysis). a: comparison to control; b: comparison to DMSO (0.166%); c: comparison to Eth (0.104%); d: comparison to “DMSO (0.166%) +Eth (0.104%)”; e: comparison to α-tocopherol (400 μM); f: comparison to SMSO (100 μg/mL); g: comparison to SESO (100μg/mL); h: comparison to SMSO (100 μg/mL); i: comparison to 7KC (62.5 μM); j: comparison to “7KC + α-tocopherol (400 μM)”; k: comparison to “7KC + SMSO (100 μg/mL)”; l: comparison to “7KC + SESO (100 μg/mL)”; m: comparison to “7KC + SMCSO (100 μg/mL)”; n: comparison to 7β-OH (62.5 μM); o: comparison to “7β-OH + α-tocopherol (400 μM)”; p: comparison to “7β-OH + SMSO (100 μg/mL)”; q: comparison to “7β-OH + SESO (100 μg/mL)”; r: comparison to “7β-OH + SMCSO (100 μg/mL)”.
    Figure Legend Snippet: THP-1 cells were incubated for 24 h with or without 7KC et 7β-OH (62.5μM) in the presence or absence of Silybum seed oils (SMSO, SESO, and SMCSO) (100 μg/mL) or α-tocopherol (400 μM). The histogram represented the percentage of DHE-positive cells. The results are expressed as mean ±SD of three independent experiments. Different letters indicate significate difference between the groups ( p <0.05, Tukey test, multiple comparative analysis). a: comparison to control; b: comparison to DMSO (0.166%); c: comparison to Eth (0.104%); d: comparison to “DMSO (0.166%) +Eth (0.104%)”; e: comparison to α-tocopherol (400 μM); f: comparison to SMSO (100 μg/mL); g: comparison to SESO (100μg/mL); h: comparison to SMSO (100 μg/mL); i: comparison to 7KC (62.5 μM); j: comparison to “7KC + α-tocopherol (400 μM)”; k: comparison to “7KC + SMSO (100 μg/mL)”; l: comparison to “7KC + SESO (100 μg/mL)”; m: comparison to “7KC + SMCSO (100 μg/mL)”; n: comparison to 7β-OH (62.5 μM); o: comparison to “7β-OH + α-tocopherol (400 μM)”; p: comparison to “7β-OH + SMSO (100 μg/mL)”; q: comparison to “7β-OH + SESO (100 μg/mL)”; r: comparison to “7β-OH + SMCSO (100 μg/mL)”.

    Techniques Used: Incubation, Comparison

    Principal component analysis (PCA) (A) and heatmap (B) of lipid profiles (fatty acids, phytosterols, and tocopherols), phenolic profiles (total phenol, flavonoid, and carotenoid contents), and antioxidant activities (DPPH, FRAP, TOA, and KRL) of the three oils. TPC: total phenolic content; Flav: total flavonoid content; Cart: total carotenoid content; TOA: total antioxidant activity; KRL (G): garlic acid equivalence; KRL (T): Trolox equivalent; δ-toco: δ-tocopherol; γ-toco: γ-tocopherol; α-toco: α-tocopherol; ∑Toco: total tocopherol content; C16:0: palmitic acid; C18:0: stearic acid; ∑UFA: unsaturated fatty acids; C18:1n-9: oleic acid; ∑MUFA: mono-unsaturated fatty acids; C18:2 n-6: linoleic acid; ∑PUFA: polyunsaturated fatty acid; ∑UFA: unsaturated fatty acids; ∑phyto: total phytosterol content; SMSO: S . marianum seed oil; SESO: S . eburneum seed oil; SMCSO: S marianum commercial seed oil.
    Figure Legend Snippet: Principal component analysis (PCA) (A) and heatmap (B) of lipid profiles (fatty acids, phytosterols, and tocopherols), phenolic profiles (total phenol, flavonoid, and carotenoid contents), and antioxidant activities (DPPH, FRAP, TOA, and KRL) of the three oils. TPC: total phenolic content; Flav: total flavonoid content; Cart: total carotenoid content; TOA: total antioxidant activity; KRL (G): garlic acid equivalence; KRL (T): Trolox equivalent; δ-toco: δ-tocopherol; γ-toco: γ-tocopherol; α-toco: α-tocopherol; ∑Toco: total tocopherol content; C16:0: palmitic acid; C18:0: stearic acid; ∑UFA: unsaturated fatty acids; C18:1n-9: oleic acid; ∑MUFA: mono-unsaturated fatty acids; C18:2 n-6: linoleic acid; ∑PUFA: polyunsaturated fatty acid; ∑UFA: unsaturated fatty acids; ∑phyto: total phytosterol content; SMSO: S . marianum seed oil; SESO: S . eburneum seed oil; SMCSO: S marianum commercial seed oil.

    Techniques Used: Antioxidant Activity Assay

    Related Articles

    Incubation:

    Article Title: Antioxidant Properties and Cytoprotective Effect of Pistacia lentiscus L. Seed Oil against 7β-Hydroxycholesterol-Induced Toxicity in C2C12 Myoblasts: Reduction in Oxidative Stress, Mitochondrial and Peroxisomal Dysfunctions and Attenuation of Cell Death
    Article Snippet: An α-tocopherol (the major component of vitamin E; Sigma-Aldrich) solution was prepared to 80 mM in absolute ethanol, as previously described [ ], and stored in the dark at 4 °C.

    Article Title: Serum retinol, β-carotene, and α-tocopherol as biomarkers for disease risk and milk production in periparturient dairy cows.
    Article Snippet: Briefly, 5-point calibration curves were constructed using stock β-carotene standard (absorbance of 1.29 AU at 450 nm; Sigma Aldrich, St. Louis, MO), retinol standard (absorbance of 0.5153 AU at 325 nm; Sigma Aldrich), and α-tocopherol solution (absorbance of 1.29 AU at 292 nm; Sigma Aldrich).

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests
    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.

    Comparison:

    Article Title: Antioxidant Properties and Cytoprotective Effect of Pistacia lentiscus L. Seed Oil against 7β-Hydroxycholesterol-Induced Toxicity in C2C12 Myoblasts: Reduction in Oxidative Stress, Mitochondrial and Peroxisomal Dysfunctions and Attenuation of Cell Death
    Article Snippet: An α-tocopherol (the major component of vitamin E; Sigma-Aldrich) solution was prepared to 80 mM in absolute ethanol, as previously described [ ], and stored in the dark at 4 °C.

    Article Title: Serum retinol, β-carotene, and α-tocopherol as biomarkers for disease risk and milk production in periparturient dairy cows.
    Article Snippet: Briefly, 5-point calibration curves were constructed using stock β-carotene standard (absorbance of 1.29 AU at 450 nm; Sigma Aldrich, St. Louis, MO), retinol standard (absorbance of 0.5153 AU at 325 nm; Sigma Aldrich), and α-tocopherol solution (absorbance of 1.29 AU at 292 nm; Sigma Aldrich).

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests
    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.

    Antioxidant Activity Assay:

    Article Title: Antioxidant Properties and Cytoprotective Effect of Pistacia lentiscus L. Seed Oil against 7β-Hydroxycholesterol-Induced Toxicity in C2C12 Myoblasts: Reduction in Oxidative Stress, Mitochondrial and Peroxisomal Dysfunctions and Attenuation of Cell Death
    Article Snippet: An α-tocopherol (the major component of vitamin E; Sigma-Aldrich) solution was prepared to 80 mM in absolute ethanol, as previously described [ ], and stored in the dark at 4 °C.

    Article Title: Serum retinol, β-carotene, and α-tocopherol as biomarkers for disease risk and milk production in periparturient dairy cows.
    Article Snippet: Briefly, 5-point calibration curves were constructed using stock β-carotene standard (absorbance of 1.29 AU at 450 nm; Sigma Aldrich, St. Louis, MO), retinol standard (absorbance of 0.5153 AU at 325 nm; Sigma Aldrich), and α-tocopherol solution (absorbance of 1.29 AU at 292 nm; Sigma Aldrich).

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests
    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.

    MTT Assay:

    Article Title: Antioxidant Properties and Cytoprotective Effect of Pistacia lentiscus L. Seed Oil against 7β-Hydroxycholesterol-Induced Toxicity in C2C12 Myoblasts: Reduction in Oxidative Stress, Mitochondrial and Peroxisomal Dysfunctions and Attenuation of Cell Death
    Article Snippet: An α-tocopherol (the major component of vitamin E; Sigma-Aldrich) solution was prepared to 80 mM in absolute ethanol, as previously described [ ], and stored in the dark at 4 °C.

    Article Title: Serum retinol, β-carotene, and α-tocopherol as biomarkers for disease risk and milk production in periparturient dairy cows.
    Article Snippet: Briefly, 5-point calibration curves were constructed using stock β-carotene standard (absorbance of 1.29 AU at 450 nm; Sigma Aldrich, St. Louis, MO), retinol standard (absorbance of 0.5153 AU at 325 nm; Sigma Aldrich), and α-tocopherol solution (absorbance of 1.29 AU at 292 nm; Sigma Aldrich).

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests
    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.

    Flow Cytometry:

    Article Title: Antioxidant Properties and Cytoprotective Effect of Pistacia lentiscus L. Seed Oil against 7β-Hydroxycholesterol-Induced Toxicity in C2C12 Myoblasts: Reduction in Oxidative Stress, Mitochondrial and Peroxisomal Dysfunctions and Attenuation of Cell Death
    Article Snippet: An α-tocopherol (the major component of vitamin E; Sigma-Aldrich) solution was prepared to 80 mM in absolute ethanol, as previously described [ ], and stored in the dark at 4 °C.

    Article Title: Serum retinol, β-carotene, and α-tocopherol as biomarkers for disease risk and milk production in periparturient dairy cows.
    Article Snippet: Briefly, 5-point calibration curves were constructed using stock β-carotene standard (absorbance of 1.29 AU at 450 nm; Sigma Aldrich, St. Louis, MO), retinol standard (absorbance of 0.5153 AU at 325 nm; Sigma Aldrich), and α-tocopherol solution (absorbance of 1.29 AU at 292 nm; Sigma Aldrich).

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests
    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.

    Staining:

    Article Title: Antioxidant Properties and Cytoprotective Effect of Pistacia lentiscus L. Seed Oil against 7β-Hydroxycholesterol-Induced Toxicity in C2C12 Myoblasts: Reduction in Oxidative Stress, Mitochondrial and Peroxisomal Dysfunctions and Attenuation of Cell Death
    Article Snippet: An α-tocopherol (the major component of vitamin E; Sigma-Aldrich) solution was prepared to 80 mM in absolute ethanol, as previously described [ ], and stored in the dark at 4 °C.

    Article Title: Serum retinol, β-carotene, and α-tocopherol as biomarkers for disease risk and milk production in periparturient dairy cows.
    Article Snippet: Briefly, 5-point calibration curves were constructed using stock β-carotene standard (absorbance of 1.29 AU at 450 nm; Sigma Aldrich, St. Louis, MO), retinol standard (absorbance of 0.5153 AU at 325 nm; Sigma Aldrich), and α-tocopherol solution (absorbance of 1.29 AU at 292 nm; Sigma Aldrich).

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests
    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.

    Activity Assay:

    Article Title: Antioxidant Properties and Cytoprotective Effect of Pistacia lentiscus L. Seed Oil against 7β-Hydroxycholesterol-Induced Toxicity in C2C12 Myoblasts: Reduction in Oxidative Stress, Mitochondrial and Peroxisomal Dysfunctions and Attenuation of Cell Death
    Article Snippet: An α-tocopherol (the major component of vitamin E; Sigma-Aldrich) solution was prepared to 80 mM in absolute ethanol, as previously described [ ], and stored in the dark at 4 °C.

    Article Title: Serum retinol, β-carotene, and α-tocopherol as biomarkers for disease risk and milk production in periparturient dairy cows.
    Article Snippet: Briefly, 5-point calibration curves were constructed using stock β-carotene standard (absorbance of 1.29 AU at 450 nm; Sigma Aldrich, St. Louis, MO), retinol standard (absorbance of 0.5153 AU at 325 nm; Sigma Aldrich), and α-tocopherol solution (absorbance of 1.29 AU at 292 nm; Sigma Aldrich).

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests
    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.

    Expressing:

    Article Title: Antioxidant Properties and Cytoprotective Effect of Pistacia lentiscus L. Seed Oil against 7β-Hydroxycholesterol-Induced Toxicity in C2C12 Myoblasts: Reduction in Oxidative Stress, Mitochondrial and Peroxisomal Dysfunctions and Attenuation of Cell Death
    Article Snippet: An α-tocopherol (the major component of vitamin E; Sigma-Aldrich) solution was prepared to 80 mM in absolute ethanol, as previously described [ ], and stored in the dark at 4 °C.

    Article Title: Serum retinol, β-carotene, and α-tocopherol as biomarkers for disease risk and milk production in periparturient dairy cows.
    Article Snippet: Briefly, 5-point calibration curves were constructed using stock β-carotene standard (absorbance of 1.29 AU at 450 nm; Sigma Aldrich, St. Louis, MO), retinol standard (absorbance of 0.5153 AU at 325 nm; Sigma Aldrich), and α-tocopherol solution (absorbance of 1.29 AU at 292 nm; Sigma Aldrich).

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests
    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.

    Microscopy:

    Article Title: Antioxidant Properties and Cytoprotective Effect of Pistacia lentiscus L. Seed Oil against 7β-Hydroxycholesterol-Induced Toxicity in C2C12 Myoblasts: Reduction in Oxidative Stress, Mitochondrial and Peroxisomal Dysfunctions and Attenuation of Cell Death
    Article Snippet: An α-tocopherol (the major component of vitamin E; Sigma-Aldrich) solution was prepared to 80 mM in absolute ethanol, as previously described [ ], and stored in the dark at 4 °C.

    Article Title: Serum retinol, β-carotene, and α-tocopherol as biomarkers for disease risk and milk production in periparturient dairy cows.
    Article Snippet: Briefly, 5-point calibration curves were constructed using stock β-carotene standard (absorbance of 1.29 AU at 450 nm; Sigma Aldrich, St. Louis, MO), retinol standard (absorbance of 0.5153 AU at 325 nm; Sigma Aldrich), and α-tocopherol solution (absorbance of 1.29 AU at 292 nm; Sigma Aldrich).

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests
    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.

    Gas Chromatography-Mass Spectrometry:

    Article Title: Antioxidant Properties and Cytoprotective Effect of Pistacia lentiscus L. Seed Oil against 7β-Hydroxycholesterol-Induced Toxicity in C2C12 Myoblasts: Reduction in Oxidative Stress, Mitochondrial and Peroxisomal Dysfunctions and Attenuation of Cell Death
    Article Snippet: An α-tocopherol (the major component of vitamin E; Sigma-Aldrich) solution was prepared to 80 mM in absolute ethanol, as previously described [ ], and stored in the dark at 4 °C.

    Article Title: Serum retinol, β-carotene, and α-tocopherol as biomarkers for disease risk and milk production in periparturient dairy cows.
    Article Snippet: Briefly, 5-point calibration curves were constructed using stock β-carotene standard (absorbance of 1.29 AU at 450 nm; Sigma Aldrich, St. Louis, MO), retinol standard (absorbance of 0.5153 AU at 325 nm; Sigma Aldrich), and α-tocopherol solution (absorbance of 1.29 AU at 292 nm; Sigma Aldrich).

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests
    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.

    Quantitative RT-PCR:

    Article Title: Antioxidant Properties and Cytoprotective Effect of Pistacia lentiscus L. Seed Oil against 7β-Hydroxycholesterol-Induced Toxicity in C2C12 Myoblasts: Reduction in Oxidative Stress, Mitochondrial and Peroxisomal Dysfunctions and Attenuation of Cell Death
    Article Snippet: An α-tocopherol (the major component of vitamin E; Sigma-Aldrich) solution was prepared to 80 mM in absolute ethanol, as previously described [ ], and stored in the dark at 4 °C.

    Article Title: Serum retinol, β-carotene, and α-tocopherol as biomarkers for disease risk and milk production in periparturient dairy cows.
    Article Snippet: Briefly, 5-point calibration curves were constructed using stock β-carotene standard (absorbance of 1.29 AU at 450 nm; Sigma Aldrich, St. Louis, MO), retinol standard (absorbance of 0.5153 AU at 325 nm; Sigma Aldrich), and α-tocopherol solution (absorbance of 1.29 AU at 292 nm; Sigma Aldrich).

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests
    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.

    Transmission Assay:

    Article Title: Antioxidant Properties and Cytoprotective Effect of Pistacia lentiscus L. Seed Oil against 7β-Hydroxycholesterol-Induced Toxicity in C2C12 Myoblasts: Reduction in Oxidative Stress, Mitochondrial and Peroxisomal Dysfunctions and Attenuation of Cell Death
    Article Snippet: An α-tocopherol (the major component of vitamin E; Sigma-Aldrich) solution was prepared to 80 mM in absolute ethanol, as previously described [ ], and stored in the dark at 4 °C.

    Article Title: Serum retinol, β-carotene, and α-tocopherol as biomarkers for disease risk and milk production in periparturient dairy cows.
    Article Snippet: Briefly, 5-point calibration curves were constructed using stock β-carotene standard (absorbance of 1.29 AU at 450 nm; Sigma Aldrich, St. Louis, MO), retinol standard (absorbance of 0.5153 AU at 325 nm; Sigma Aldrich), and α-tocopherol solution (absorbance of 1.29 AU at 292 nm; Sigma Aldrich).

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests
    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.

    Electron Microscopy:

    Article Title: Antioxidant Properties and Cytoprotective Effect of Pistacia lentiscus L. Seed Oil against 7β-Hydroxycholesterol-Induced Toxicity in C2C12 Myoblasts: Reduction in Oxidative Stress, Mitochondrial and Peroxisomal Dysfunctions and Attenuation of Cell Death
    Article Snippet: An α-tocopherol (the major component of vitamin E; Sigma-Aldrich) solution was prepared to 80 mM in absolute ethanol, as previously described [ ], and stored in the dark at 4 °C.

    Article Title: Serum retinol, β-carotene, and α-tocopherol as biomarkers for disease risk and milk production in periparturient dairy cows.
    Article Snippet: Briefly, 5-point calibration curves were constructed using stock β-carotene standard (absorbance of 1.29 AU at 450 nm; Sigma Aldrich, St. Louis, MO), retinol standard (absorbance of 0.5153 AU at 325 nm; Sigma Aldrich), and α-tocopherol solution (absorbance of 1.29 AU at 292 nm; Sigma Aldrich).

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests
    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.

    Cell Culture:

    Article Title: Antioxidant Properties and Cytoprotective Effect of Pistacia lentiscus L. Seed Oil against 7β-Hydroxycholesterol-Induced Toxicity in C2C12 Myoblasts: Reduction in Oxidative Stress, Mitochondrial and Peroxisomal Dysfunctions and Attenuation of Cell Death
    Article Snippet: An α-tocopherol (the major component of vitamin E; Sigma-Aldrich) solution was prepared to 80 mM in absolute ethanol, as previously described [ ], and stored in the dark at 4 °C.

    Article Title: Serum retinol, β-carotene, and α-tocopherol as biomarkers for disease risk and milk production in periparturient dairy cows.
    Article Snippet: Briefly, 5-point calibration curves were constructed using stock β-carotene standard (absorbance of 1.29 AU at 450 nm; Sigma Aldrich, St. Louis, MO), retinol standard (absorbance of 0.5153 AU at 325 nm; Sigma Aldrich), and α-tocopherol solution (absorbance of 1.29 AU at 292 nm; Sigma Aldrich).

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests
    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.



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    four-point standard working solution of vitamin e (α-tocopherol) (chromatography grade  (Merck KGaA)
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    Peroxide value, free fatty acid, slip melting point and <t> vitamin E </t> in DPL.
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    standard working solution of vitamin e (α-tocopherol)  (Merck KGaA)
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    Image Search Results


    THP-1 cells were incubated for 24 h with or without 7KC et 7β-OH (62.5μM) in the presence or absence of Silybum seed oils (SMSO, SESO, and SMCSO) (100 μg/mL) or α-tocopherol (400 μM). The histogram represented the percentage of DHE-positive cells. The results are expressed as mean ±SD of three independent experiments. Different letters indicate significate difference between the groups ( p <0.05, Tukey test, multiple comparative analysis). a: comparison to control; b: comparison to DMSO (0.166%); c: comparison to Eth (0.104%); d: comparison to “DMSO (0.166%) +Eth (0.104%)”; e: comparison to α-tocopherol (400 μM); f: comparison to SMSO (100 μg/mL); g: comparison to SESO (100μg/mL); h: comparison to SMSO (100 μg/mL); i: comparison to 7KC (62.5 μM); j: comparison to “7KC + α-tocopherol (400 μM)”; k: comparison to “7KC + SMSO (100 μg/mL)”; l: comparison to “7KC + SESO (100 μg/mL)”; m: comparison to “7KC + SMCSO (100 μg/mL)”; n: comparison to 7β-OH (62.5 μM); o: comparison to “7β-OH + α-tocopherol (400 μM)”; p: comparison to “7β-OH + SMSO (100 μg/mL)”; q: comparison to “7β-OH + SESO (100 μg/mL)”; r: comparison to “7β-OH + SMCSO (100 μg/mL)”.

    Journal: PLOS ONE

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests

    doi: 10.1371/journal.pone.0304021

    Figure Lengend Snippet: THP-1 cells were incubated for 24 h with or without 7KC et 7β-OH (62.5μM) in the presence or absence of Silybum seed oils (SMSO, SESO, and SMCSO) (100 μg/mL) or α-tocopherol (400 μM). The histogram represented the percentage of DHE-positive cells. The results are expressed as mean ±SD of three independent experiments. Different letters indicate significate difference between the groups ( p <0.05, Tukey test, multiple comparative analysis). a: comparison to control; b: comparison to DMSO (0.166%); c: comparison to Eth (0.104%); d: comparison to “DMSO (0.166%) +Eth (0.104%)”; e: comparison to α-tocopherol (400 μM); f: comparison to SMSO (100 μg/mL); g: comparison to SESO (100μg/mL); h: comparison to SMSO (100 μg/mL); i: comparison to 7KC (62.5 μM); j: comparison to “7KC + α-tocopherol (400 μM)”; k: comparison to “7KC + SMSO (100 μg/mL)”; l: comparison to “7KC + SESO (100 μg/mL)”; m: comparison to “7KC + SMCSO (100 μg/mL)”; n: comparison to 7β-OH (62.5 μM); o: comparison to “7β-OH + α-tocopherol (400 μM)”; p: comparison to “7β-OH + SMSO (100 μg/mL)”; q: comparison to “7β-OH + SESO (100 μg/mL)”; r: comparison to “7β-OH + SMCSO (100 μg/mL)”.

    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.

    Techniques: Incubation, Comparison

    Principal component analysis (PCA) (A) and heatmap (B) of lipid profiles (fatty acids, phytosterols, and tocopherols), phenolic profiles (total phenol, flavonoid, and carotenoid contents), and antioxidant activities (DPPH, FRAP, TOA, and KRL) of the three oils. TPC: total phenolic content; Flav: total flavonoid content; Cart: total carotenoid content; TOA: total antioxidant activity; KRL (G): garlic acid equivalence; KRL (T): Trolox equivalent; δ-toco: δ-tocopherol; γ-toco: γ-tocopherol; α-toco: α-tocopherol; ∑Toco: total tocopherol content; C16:0: palmitic acid; C18:0: stearic acid; ∑UFA: unsaturated fatty acids; C18:1n-9: oleic acid; ∑MUFA: mono-unsaturated fatty acids; C18:2 n-6: linoleic acid; ∑PUFA: polyunsaturated fatty acid; ∑UFA: unsaturated fatty acids; ∑phyto: total phytosterol content; SMSO: S . marianum seed oil; SESO: S . eburneum seed oil; SMCSO: S marianum commercial seed oil.

    Journal: PLOS ONE

    Article Title: Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests

    doi: 10.1371/journal.pone.0304021

    Figure Lengend Snippet: Principal component analysis (PCA) (A) and heatmap (B) of lipid profiles (fatty acids, phytosterols, and tocopherols), phenolic profiles (total phenol, flavonoid, and carotenoid contents), and antioxidant activities (DPPH, FRAP, TOA, and KRL) of the three oils. TPC: total phenolic content; Flav: total flavonoid content; Cart: total carotenoid content; TOA: total antioxidant activity; KRL (G): garlic acid equivalence; KRL (T): Trolox equivalent; δ-toco: δ-tocopherol; γ-toco: γ-tocopherol; α-toco: α-tocopherol; ∑Toco: total tocopherol content; C16:0: palmitic acid; C18:0: stearic acid; ∑UFA: unsaturated fatty acids; C18:1n-9: oleic acid; ∑MUFA: mono-unsaturated fatty acids; C18:2 n-6: linoleic acid; ∑PUFA: polyunsaturated fatty acid; ∑UFA: unsaturated fatty acids; ∑phyto: total phytosterol content; SMSO: S . marianum seed oil; SESO: S . eburneum seed oil; SMCSO: S marianum commercial seed oil.

    Article Snippet: Similarly, a α-tocopherol solution (Sigma-Aldrich) was prepared at 80 mM in absolute ethanol, and stored in the dark at 4°C.

    Techniques: Antioxidant Activity Assay

    Chemical structure of α-, β-, γ-, and δ-tocopherols and tocotrienols (based on ).

    Journal: Foods

    Article Title: Obtaining Bixin- and Tocotrienol-Rich Extracts from Peruvian Annatto Seeds Using Supercritical CO 2 Extraction: Experimental and Economic Evaluation

    doi: 10.3390/foods13101549

    Figure Lengend Snippet: Chemical structure of α-, β-, γ-, and δ-tocopherols and tocotrienols (based on ).

    Article Snippet: A standard mixed solution of α-, β-, γ-, and δ-tocopherols and tocotrienols was purchased from Chromadex Company (Los Angeles, CA, USA).

    Techniques:

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Central FGF21 production regulates memory but not peripheral metabolism

    doi: 10.1016/j.celrep.2022.111239

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: (±)–α–Tocopherol (Vitamin E) solution , Sigma , Cat#: V-020–1ML.

    Techniques: Virus, Recombinant, Expressing, Software, RNAscope, Multiplex Assay, Plasmid Preparation, Reverse Transcription

    Peroxide value, free fatty acid, slip melting point and vitamin E in DPL.

    Journal: Molecules

    Article Title: Preliminary Evaluation of Supercritical Carbon Dioxide Extracted Dabai Pulp Oleoresin as a New Alternative Fat

    doi: 10.3390/molecules26185545

    Figure Lengend Snippet: Peroxide value, free fatty acid, slip melting point and vitamin E in DPL.

    Article Snippet: The quantitative assay was achieved using an external calibration curve of the four-point standard working solution of vitamin E (α-tocopherol) (Chromatography grade; Merck KGaA, Darmstadt, Germany).

    Techniques: